Analysis of the Interaction between Eglin C and 2709 Alkaline Protease and Establishment of an Affinity Purification Method / 中国生物化学与分子生物学报

ABSTRACT

Eglin C is a small heat-stabilized protein from leeches. It belongs to the potato chymotrypsin inhibitor family, and can inhibit elastase, subtilisin, cathepsin, α-lytic protease and chymotrypsin etc. However, purification of protease by eglin C has not been studied. In this paper, the cDNA sequences encoding eglin C and its mutants were chemically synthesized and cloned into the prokaryotic expression vector pQE30. His