Expression and clinical significance of lncRNA-Xist in lumbar disc degeneration nucleus pulposus tissue / 国际生物医学工程杂志

ABSTRACT

Objective:To explore the expression and clinical significance of long non-coding RNA (lncRNA) Xist in the nucleus pulposus tissue of lumbar intervertebral disc degeneration (LIDD).Methods:The Trizol extraction method was used to extract total RNA from LIDD nucleus pulposus tissue cells and in vitro cultured nucleus pulposus cells. The relative expression level of lncRNA Xist in LIDD nucleus pulposus tissue was verified through real-time fluorescence quantitative PCR. In vitro, a knockdown expression model was constructed by transfecting si-lncRNA Xist into a nucleus pulposus tissue cell line. The knockdown efficiency was identified using real-time fluorescence quantitative PCR. Cell proliferation behavior was detected using EdU staining and flow cytometry. Western Blot and flow cytometry were used to detect cell apoptosis. Results:Compared with control group, lncRNA Xist expression in LIDD nucleus pulposus tissues was increased ( P < 0.01). Compared with normal group, the number of EdU positive cells and the number of S phase cells in TNF-α group were decreased (all P < 0.01), cell apoptosis were increased ( P < 0.01), Bax protein expression was increased ( P < 0.01), and Bcl-2 protein expression was decreased ( P < 0.05). Compared with TNF-α group, the number of EdU positive cells and the number of S phase cells in TNF-α + siRNA-lncRNA Xist group were increased (all P < 0.05), cell apoptosis were decreased ( P < 0.05), Bax protein expression was decreased, and Bcl-2 protein expression was increased. Conclusions:lncRNA Xist is highly expressed in nucleus pulposus tissues of LIDD patients, inhibits the proliferation of nucleus pulposus cells, and promotes apoptosis, which suggests that lncRNA Xist may be used as a therapeutic target and biomarker of LIDD.