Discovery of the serum biomarker proteins in severe preeclampsia by proteomic analysis
Experimental & Molecular Medicine
;
: 427-435, 2011.
Artículo
en Inglés
| WPRIM
| ID: wpr-102680
ABSTRACT
Preeclapsia (PE) is a severe disorder that occurs during pregnancy, leading to maternal and fetal morbidity and mortality. PE affects about 3-8% of all pregnancies. In this study, we conducted liquid chromatographymass spectrometry/mass spectrometry (LC-MS/MS) to analyze serum samples depleted of the six most abundant proteins from normal and PE-affected pregnancies to profile serum proteins. A total of 237 proteins were confidently identified with < 1% false discovery rate from the two groups of duplicate analysis. The expression levels of those identified proteins were compared semiquantitatively by spectral counting. To further validate the candidate proteins with a quantitative mass spectrometric method, selective reaction monitoring (SRM) and enzyme linked immune assay (ELISA) of serum samples collected from pregnant women with severe PE (n = 8) or normal pregnant women (n = 5) was conducted. alpha2-HS-glycoprotein (AHSG), retinol binding protein 4 (RBP4) and alpha-1-microglobulin/bikunin (AMBP) and Insulin like growth factor binding protein, acid labile subunit (IGFBP-ALS) were confirmed to be differentially expressed in PE using SRM (P < 0.05). Among these proteins, AHSG was verified by ELISA and showed a statistically significant increase in PE samples when compared to controls.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Preeclampsia
/
Alfa-Globulinas
/
Datos de Secuencia Molecular
/
Proteínas Sanguíneas
/
Biomarcadores
/
Estudios de Casos y Controles
/
Secuencia de Aminoácidos
/
Proteínas de Unión a Factor de Crecimiento Similar a la Insulina
/
Proteoma
/
Proteínas Plasmáticas de Unión al Retinol
Tipo de estudio:
Estudio observacional
/
Estudio pronóstico
Límite:
Adulto
/
Femenino
/
Humanos
/
Embarazo
Idioma:
Inglés
Revista:
Experimental & Molecular Medicine
Año:
2011
Tipo del documento:
Artículo
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