The role of promoter methylation in Epstein-Barr virus (EBV) microRNA expression in EBV-infected B cell lines
Experimental & Molecular Medicine
;
: 401-410, 2011.
Artículo
en Inglés
| WPRIM
| ID: wpr-102683
ABSTRACT
Epstein-Barr virus (EBV) microRNAs (miRNAs) are expressed in EBV-associated tumors and cell lines, but the regulation mechanism of their expression is unclear yet. We investigated whether the expression of EBV miRNAs is epigenetically regulated in EBV-infected B cell lines. The expression of BART miRNAs was inversely related with the methylation level of the BART promoter at both steady-state and following 5-aza-2'-deoxycytidine treatment of the cells. The expression of BHRF1 miRNAs also became detectable with the demethylation of Cp/Wp in latency I EBV-infected cell lines. Furthermore, in vitro methylation of the BART and Cp promoters reduced the promoter-driven transactivation. In contrast, tricostatin A had little effect on the expression of EBV miRNA expression as well as on the BART and Cp/Wp promoters. Our results suggest that promoter methylation, but not histone acetylation, plays a role in regulation of the EBV miRNA expression in EBV-infected B cell lines.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Azacitidina
/
Proteínas Virales
/
ARN Viral
/
Linfocitos B
/
Metilasas de Modificación del ADN
/
Regulación Viral de la Expresión Génica
/
Línea Celular
/
Regiones Promotoras Genéticas
/
Herpesvirus Humano 4
/
Metilación de ADN
Límite:
Humanos
Idioma:
Inglés
Revista:
Experimental & Molecular Medicine
Año:
2011
Tipo del documento:
Artículo
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