Inhibition of NHE-1 mRNA expression in penumbra area by human interleukin-10 transfection following cerebral ischemia-reperfusion injury in rats / 中华神经医学杂志

ABSTRACT

Objective To explore the effect of SA liposome mediated human interleukin-10 (IL-10) gent transfection on NHE-1 mRNA expression in penumbra area following focal cerebral ischemia reperfusion injury in rats. Methods Totally 78 male SD rats were randomly divided into normal control group (n=6), MCAO group (n=24), hIL-10 transfection group (n=24) and empty vector transfection group (n=24). Longa's method was employed to establish MCAO models in the latter 3 groups. The rats in the MCAO group underwent stereotactic operation without drug injection, and the hIL-10 transfection group and empty vector transfection group were injected stereotactieally with pcDNA3.1-IL-10 and pcDNA3.1, respectively, both by SA liposome mediation. After transfection, RT-PCR and ELISA were used to determine the effect of transfection, TTC staining was conducted to detect the infarct volume. Meanwhile, real-time quantitative PCR was performed to examine the expressions of NHE-1 mRNA and NF-κB mRNA in the penumbra area. Results (1) SA liposome effectively mediated the hIL-10 gene to transfect the brain tissue. Also hIL-10 gene transfection played neuroprotective effect by reducing the brain infarct volume. (2) The expression of NF-κB mRNA in different groups was 1.00±0.33, 4.76±0.41, 4.58±0.62 and 2.77±0.43, respectively, hIL-10 gene transfection also inhibited the increase of NF-κB mRNA expression in the penumbra area following the cerebral ischemia reperfusion injury. (3) The expression of NHE-1mRNA was 1.00±0.22, 4.16±0.48, 3.97±0.51 and 2.82±0.47, respectively, hIL-10 gene transfection also inhibited the increase of NHE-1 mRNA expression in the penumbra area following the cerebral ischemia reperfusion injury. Conclusions The hIL-10 transfection can exert the protective effect on the brain against cerebral ischemia-reperfusion injury partly via inhibiting the NHE-1 mRNA expression.