Isolation and Partial Characterization of a 50 kDa Hemin-regulated Cell Envelope Protein from Prevotella nigrescens / 대한치주과학회지
The Journal of the Korean Academy of Periodontology
;
: 351-360, 2002.
Artículo
en Coreano
| WPRIM
| ID: wpr-103307
ABSTRACT
In the study presented here, identification, purification, and partial characterization of a hemin-regulated protein in Prevotella nigrescens were carried out. The results of this study confirm that the availability of hemin influences the expression of a selected membrane protein as well as the growth rate of P. nigrescens ATCC 33563. The 50 kDa cell envelope associated protein, whose expression is hemin regulated, is considered to be a putative hemin-binding protein from P. nigrescens. Disulfide bonds were not present in this protein, and N'-terminal amino acid sequence analysis revealed that this protein belongs to a new, so far undescribed protein. The 50 kDa protein was found to be rich in hydrophilic amino acids, with glycine comprising approximately 60% of the total amino acids. The study described here is the first to identify, purify, and biochemically characterize a putative hemin-binding protein from P. nigrescens. Work is in progress to further characterize the molecular structure of this protein.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Estructura Molecular
/
Prevotella
/
Análisis de Secuencia de Proteína
/
Prevotella nigrescens
/
Aminoácidos
/
Glicina
/
Hemina
/
Proteínas de la Membrana
Idioma:
Coreano
Revista:
The Journal of the Korean Academy of Periodontology
Año:
2002
Tipo del documento:
Artículo
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