Effect of salvianolic acid B on activated platelet-induced inflammatory response in human brain micro-vascular endothelial cells / 中华神经医学杂志

ABSTRACT

Objective To investigate the interventional effect ofsalvianolic acid B(SalB)on activated platelet-induced inflammatory response in human brain microvascular endothelial cells (HBMECs).Methods Activated platelets and HBMECs were co-cultured to establish inflammatory response models.The mRNA expression levels of inflammatory mediators,including intercellular adhesion molecule-1(ICAM-1),IL-1β,IL-6,IL-8 and monocyte chemoattractant protein-1(MCP-1)in HBMECs,were detected by quantitative RT-PCR.Cells of the pretreatment group were pretreated with 10 μg/ml SalB for 24 h,and the changes of mRNA expression levels of these inflammatory mediators were analyzed.Results The mRNA expression levels ofICAM-1,IL-1β,IL-6,IL-8 and MCP-1 in activated platelet-induced inflammatory response model of SalB pretreatnent group were significantly reduced as compared with those without pretreatment(P＜0.05).Conclusion SalB can repress the activated platelet-induced inflammatory response in HBMECs,which might be one of the mechanisms of anti-cerebral ischemia effect.