Identification of a putative cellular receptor 150 kDa polypeptide for porcine epidemic diarrhea virus in porcine enterocytes
Journal of Veterinary Science
;
: 269-275, 2003.
Artículo
en Inglés
| WPRIM
| ID: wpr-103630
ABSTRACT
Porcine epidemic diarrhea virus (PEDV) causes an acute enteritis in pigs of all ages, often fatality for neonates. PEDV occupies an intermediate position between two well characterized members of the coronavirus group I, human coronavirus (HCoV-229E)and transmissible gastroenteritis virus (TGEV) which uses aminopeptidase N (APN), a 150 kDa protein, as their receptors. However, the receptor of the PEDV has not been identified yet. A virus overlay protein binding assay (VOPBA) was used to identify PEDV binding protein in permissive cells. The binding ability of PEDV to porcine APN (pAPN) and the effects of pAPN on infectivity of PEDV in Vero cells were also investigated. VOPBA identified a 150 kDa protein, as a putative PEDV receptor in enterocytes and swine testicle (ST) cells. Further the PEDV binding to pAPN was blocked by anti-pAPN and pAPN enhanced PEDV infectivity in Vero cells. In conclusion, these results suggested that pAPN may act as a receptor of PEDV.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Unión Proteica
/
Receptores Virales
/
Porcinos
/
Enfermedades de los Porcinos
/
Células Vero
/
Ensayo de Inmunoadsorción Enzimática
/
Chlorocebus aethiops
/
Infecciones por Coronavirus
/
Coronavirus
/
Antígenos CD13
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2003
Tipo del documento:
Artículo
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