Effects of Heme Oxygenase-1 Expression in Mycophenolic Acid Induced Apoptosis of Jurkat Cell Lines
Journal of the Korean Surgical Society
; : 343-349, 2010.
Article
en Ko
| WPRIM
| ID: wpr-10366
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE: This study demonstrates that pharmacologic induction of heme oygenase-1 (HO-1) along with catalytic activation significantly modulated apoptosis of Jurkat cells induced by mycophenolic acid (MPA). METHODS: Cells were cultured with the presence or absence of MPA. Flow cytometric analysis was performed after propidium iodide staining. Western blotting of HO-1, Bcl, and Bax was also performed. Cells were stained 4'-6-Diamidino-2-phenylindole (DAPI) and measured by flow cytometry in the absence or presence of CoPPIX. RESULTS: Treatment of MPA decreased cell viability in a dose- and time-dependent manner. MPA-induced cell death was confirmed as apoptosis characterized by sub G0/G1 phase arrest. Expression of HO-1 assumes a pattern of decline after rising at the initial phase. CoPPIX, HO-1 inducer, significantly inhibited the cisplatin-induced apoptosis. Treatment of MPA resulted in reactive oxygen species (ROS) generation in Jurkat cells. CoPPIX attenuated ROS production in MPA-treated cells. CONCLUSION: This result suggests that the protective mechanism of HO-1 on MPA-induced cytotoxicity is associated with direct inhibition of ROS generation and mitochondrial permeability transition.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Permeabilidad
/
Propidio
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Supervivencia Celular
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Western Blotting
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Muerte Celular
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Especies Reactivas de Oxígeno
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Apoptosis
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Células Jurkat
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Hemo-Oxigenasa 1
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Citometría de Flujo
Límite:
Humans
Idioma:
Ko
Revista:
Journal of the Korean Surgical Society
Año:
2010
Tipo del documento:
Article