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Generation and Immunity Testing of a Recombinant Adenovirus Expressing NcSRS2-NcGRA7 Fusion Protein of Bovine Neospora caninum
The Korean Journal of Parasitology ; : 247-253, 2013.
Artículo en Inglés | WPRIM | ID: wpr-103945
ABSTRACT
Neospora caninum is the etiologic agent of bovine neosporosis, which affects the reproductive performance of cattle worldwide. The transmembrane protein, NcSRS2, and dense-granule protein, NcGRA7, were identified as protective antigens based on their ability to induce significant protective immune responses in murine neosporosis models. In the current study, NcSRS2 and NcGRA7 genes were spliced by overlap-extension PCR in a recombinant adenovirus termed Ad5-NcSRS2-NcGRA 7, expressing the NcSRS2-NcGRA7 gene, and the efficacy was evaluated in mice. The results showed that the titer of the recombinant adenovirus was 10(9)TCID50/ml. Three weeks post-boost immunization (w.p.b.i.), the IgG antibody titer in sera was as high as 14,096. IFN-gamma and IL-4 levels were significantly different from the control group (P<0.01). This research established a solid foundation for the development of a recombinant adenovirus vaccine against bovine N. caninum.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Proteínas Recombinantes de Fusión / Inmunoglobulina G / Proteínas Fúngicas / Portadores de Fármacos / Vacunas Fúngicas / Vacunas Sintéticas / Adenoviridae / Interleucina-4 / Interferón gamma / Neospora Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Inglés Revista: The Korean Journal of Parasitology Año: 2013 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Proteínas Recombinantes de Fusión / Inmunoglobulina G / Proteínas Fúngicas / Portadores de Fármacos / Vacunas Fúngicas / Vacunas Sintéticas / Adenoviridae / Interleucina-4 / Interferón gamma / Neospora Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Inglés Revista: The Korean Journal of Parasitology Año: 2013 Tipo del documento: Artículo