The correlation of NLRP3 with inflammatory factors and stroke prognosis in acute ischemic patients / 中风与神经疾病杂志

ABSTRACT

@#Objective　To study the correlation of NLRP3 with inflammatory factors and neurological function recovery in acute ischemic stroke (AIS) patients.Methods　Seventy-one AIS patients were enrolled,blood samples were collected at 3~72 hours after stroke onset.The mRNA levels of NLRP3 were detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR).Serum levels of inflammatory cytokines IL-6,IL-1β and TNF-α were detected by ELISA.The modified RanKin sore (mRS) was used to evaluate the recovery of AIS patients 3 months after stroke onset.Thirty-eight healthy normal people were recruited as a controls,blood samples were collected during recruitment.Results　The mRNA levels of NLRP3 in the blood of AIS patients were significantly higher than thos normal control group (P<0.05).Conclusion　Analysis showed that the NLRP3 mRNA levels were positively correlated with the mRS score (r=0.357,P=0.002).In addition,the levels of inflammation-related IL-1β,IL-6,and TNF-α in AIS patients were significantly higher than those in the normal control group,with P<0.05.The mRNA expression of NLRP3 was positively correlated with the levels of inflammatory factor IL-1β(r=0.31,P=0.016),but not significantly correlated with IL-6 (r=0.07,P=0.751) and TNF-α (r=0.14,P=0.186).The prognosis of AIS patients with higher levels of NLRP3 expression in the blood is worse.In addition,the inflammatory factor IL-1β mediates the pathogenesis of NLRP3.