Semi-Quantitative Analyses of Hippocampal Heat Shock Protein-70 Expression Based on the Duration of Ischemia and the Volume of Cerebral Infarction in Mice
Journal of Korean Neurosurgical Society
;
: 307-312, 2014.
Artículo
en Inglés
| WPRIM
| ID: wpr-104540
ABSTRACT
OBJECTIVE:
We investigated the expression of hippocampal heat shock protein 70 (HSP-70) infarction volume after different durations of experimental ischemic stroke in mice.METHODS:
Focal cerebral ischemia was induced in mice by occluding the middle cerebral artery with the modified intraluminal filament technique. Twenty-four hours after ischemia induction, both hippocampi were extracted for HSP-70 protein analyses. Slices from each hemisphere were stained with 2,3,5-triphenyltetrazolium chloride (2%), and infarction volumes were calculated. HSP-70 levels were evaluated using western blot and enzyme-linked immunosorbent assay (ELISA). HSP-70 subtype (hsp70.1, hspa1a, hspa1b) mRNA levels in the hippocampus were measured using reverse transcription-polymerase chain reaction (RT-PCR).RESULTS:
Cerebral infarctions were found ipsilateral to the occlusion in 10 mice exposed to transient ischemia (5 each in the 30-min and 60-min occlusion groups), whereas no focal infarctions were noted in any of the sham mice. The average infarct volumes of the 2 ischemic groups were 22.28+/-7.31 mm3 [30-min group+/-standard deviation (SD)] and 38.06+/-9.53 mm3 (60-min group+/-SD). Western blot analyses and ELISA showed that HSP-70 in hippocampal tissues increased in the infarction groups than in the sham group. However, differences in HSP-70 levels between the 2 infarction groups were statistically insignificant. Moreover, RT-PCR results demonstrated no relationship between the mRNA expression of HSP-70 subtypes and occlusion time or infarction volume.CONCLUSION:
Our results indicated no significant difference in HSP-70 expression between the 30- and 60-min occlusion groups despite the statistical difference in infarction volumes. Furthermore, HSP-70 subtype mRNA expression was independent of both occlusion duration and cerebral infarction volume.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Choque
/
ARN Mensajero
/
Ensayo de Inmunoadsorción Enzimática
/
Infarto Cerebral
/
Isquemia Encefálica
/
Western Blotting
/
Proteínas HSP70 de Choque Térmico
/
Arteria Cerebral Media
/
Accidente Cerebrovascular
/
Calor
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of Korean Neurosurgical Society
Año:
2014
Tipo del documento:
Artículo
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