Radiation up-regulates the expression of VEGF in a canine oral melanoma cell line
Journal of Veterinary Science
;
: 207-214, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-104697
ABSTRACT
To evaluate radiosensitivity and the effects of radiation on the expression of vascular endothelial growth factor (VEGF) and VEGF receptors in the canine oral melanoma cell line, TLM 1, cells were irradiated with doses of 0, 2, 4, 6, 8 and 10 Gray (Gy). Survival rates were then determined by a MTT assay, while vascular endothelial growth factor receptor (VEGFR)-1 and -2 expression was measured by flow cytometry and apoptotic cell death rates were investigated using an Annexin assay. Additionally, a commercially available canine VEGF ELISA kit was used to measure VEGF. Radiosensitivity was detected in TLM 1 cells, and mitotic and apoptotic cell death was found to occur in a radiation dose dependent manner. VEGF was secreted constitutively and significant up-regulation was observed in the 8 and 10 Gy irradiated cells. In addition, a minor portion of TLM 1 cells expressed vascular endothelial growth factor receptor (VEGFR)-1 intracellularly. VEGFR-2 was detected in the cytoplasm and was down-regulated following radiation with increasing dosages. In TLM 1 cells, apoptosis plays an important role in radiation induced cell death. It has also been suggested that the significantly higher VEGF production in the 8 and 10 Gy group could lead to tumour resistance.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Tolerancia a Radiación
/
Sales de Tetrazolio
/
Tiazoles
/
Ensayo de Inmunoadsorción Enzimática
/
Neoplasias de la Boca
/
Regulación hacia Arriba
/
Apoptosis
/
Receptor 1 de Factores de Crecimiento Endotelial Vascular
/
Receptor 2 de Factores de Crecimiento Endotelial Vascular
/
Línea Celular Tumoral
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2013
Tipo del documento:
Artículo
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