Granulocyte Macrophage-Colony Stimulating Factor Signaling in Development of Mouse Embryos / 대한불임학회지
Korean Journal of Fertility and Sterility
;
: 5-14, 2003.
Artículo
en Coreano
| WPRIM
| ID: wpr-105119
ABSTRACT
OBJECTIVE Present study was aimed to verify the effect of granulocyte macrophage-colony stimulating factor (GM-CSF) in the preimplantation development of mouse embryos and the involvement of the mitogen activated protein kiase (MAPK) in the GM-CSF signaling. METHODS:
Two-cell embryos were cultured for 96 h in the presence or absence of GM-CSF (0, 0.4, 2, 10 ng/ml) and PD98059, a MEK inhibitor (10 muM). Morphological development, cell number per blastocyst, and apoptotic nuclei, were eamined. MAPK activity of embryonic immunoprecipitate by MAPK (ERK1/2) antibody was measured by in vitro phosphorylation of myelin basic protein.RESULTS:
At post hCG 122 h the embryonic development among the experimental groups was significantly different (p=0.018). The rate of blastocyst development and cell number per embryo were the highest in 2 ng/ml GM-CSF treatment group. The percent of apoptotic cells of the GM-CSF-treated embryos was the lowest among the group. in blastocysts, GM-CSF treatment transiently increased MAPK activity. PD098059 attenuated the effect of GM-CSF on the morphological development, increase in cell number per blastocyst, down regulation of apoptosis, and upregulation of MAPK activity, suggesting that activation of MAPK cascade possibly mediated the embryotropic effect of GM-CSF.CONCLUSION:
This result suggested that GM-CSF potentiated the development of preimplantation mouse embryos by activation of MAPK.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fosforilación
/
Blastocisto
/
Regulación hacia Abajo
/
Recuento de Células
/
Regulación hacia Arriba
/
Factor Estimulante de Colonias de Granulocitos y Macrófagos
/
Apoptosis
/
Desarrollo Embrionario
/
Estructuras Embrionarias
/
Proteína Básica de Mielina
Límite:
Animales
/
Embarazo
Idioma:
Coreano
Revista:
Korean Journal of Fertility and Sterility
Año:
2003
Tipo del documento:
Artículo
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