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Expression of Cyclooxygenase-2 in Human Intestinal Epithelial Cells Stimulated with Bacteroides fragilis Enterotoxin
Journal of Bacteriology and Virology ; : 147-157, 2002.
Artículo en Coreano | WPRIM | ID: wpr-105399
ABSTRACT
A ~20 kDa heat-labile toxin (BFT) produced by enterotoxigenic B. fragilis induces chemokine responses that may be associated with mucosal inflammation. To determine whether epithelial cells can contribute to BFT-induced inflammation, we assessed the expression of cyclooxygenase (COX)-2 in BFT-stimulated human intestinal epithelial cells. Human intestinal epithelial cell lines, HT-29 and Caco-2, were incubated with purified BFT. COX-2 mRNA and protein expression were measured by quantitative RT-PCR and Western blot analysis, respectively. BFT increased expression of COX-2, not that of COX-1, in human intestinal epithelial cell lines. Up-regulated COX-2 expression was paralleled by increased prostaglandin E2 (PGE2) production measured by the radioimmunoassay. PGE2 was predominantly secreted from the basolateral surface of BFT-treated epithelial cells, whereas lactate dehydrogenase was released predominantly from the apical surface. Moreover, the COX-2 expression and PGE2 production were significantly suppressed when NF-kappaB activity was inhibited. The basolateral secretion of PGE2 by up-regulated COX-2 in the BFT-stimulated colon epithelial cells seems to contribute to the inflammatory response in the underlying intestinal mucosa.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Bacteroides / Bacteroides fragilis / ARN Mensajero / Radioinmunoensayo / Dinoprostona / Western Blotting / FN-kappa B / Prostaglandina-Endoperóxido Sintasas / Colon / Enterotoxinas Límite: Humanos Idioma: Coreano Revista: Journal of Bacteriology and Virology Año: 2002 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Bacteroides / Bacteroides fragilis / ARN Mensajero / Radioinmunoensayo / Dinoprostona / Western Blotting / FN-kappa B / Prostaglandina-Endoperóxido Sintasas / Colon / Enterotoxinas Límite: Humanos Idioma: Coreano Revista: Journal of Bacteriology and Virology Año: 2002 Tipo del documento: Artículo