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Molecular cloning and sequencing of rat Cdc42 GTPase cDNA
Experimental & Molecular Medicine ; : 115-119, 2000.
Artículo en Inglés | WPRIM | ID: wpr-105754
ABSTRACT
Cdc42 is a member of the Rho family of small GTP-ase and plays an important role in intracellular signaling pathways regulating cell morphology, motility and stimulation of DNA synthesis. We have isolated cDNA encoding Cdc42 from a rat brain cDNA library using PCR-cloning strategy. The sequence of isolated gene revealed an open reading frame of 576 nucleotides with the potential to encode a protein of 191 amino acids with a predicted molecular weight of 21 kD. The resulting sequence was incorporated into the GenBank with accession number, AF205635. Sequence analysis revealed that overall cDNA sequence identity is 96% with human G25K and 52% with rat Chp, a homologue of the GTPase human Cdc42Hs, and having one nucleotide difference from the mouse Cdc42. However, putative protein sequence was identical to the mouse and human brain Cdc42Hs. On expression of the cDNA in COS-7 cells, a protein molecular weight of 21 kD was detected in immunoblotting using anti-human Cdc42 antibodies. Therefore, these results suggest that the cDNA we are reporting is most likely the rat homologue of the GTPase human Cdc42.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Estudio Comparativo / Datos de Secuencia Molecular / Secuencia de Bases / Secuencia de Aminoácidos / Clonación Molecular / Homología de Secuencia de Aminoácido / Análisis de Secuencia de ADN / ADN Complementario / Proteína de Unión al GTP cdc42 / Reacciones Cruzadas Límite: Animales / Humanos Idioma: Inglés Revista: Experimental & Molecular Medicine Año: 2000 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Estudio Comparativo / Datos de Secuencia Molecular / Secuencia de Bases / Secuencia de Aminoácidos / Clonación Molecular / Homología de Secuencia de Aminoácido / Análisis de Secuencia de ADN / ADN Complementario / Proteína de Unión al GTP cdc42 / Reacciones Cruzadas Límite: Animales / Humanos Idioma: Inglés Revista: Experimental & Molecular Medicine Año: 2000 Tipo del documento: Artículo