Effect of Nitric Oxide on the Trabecular Meshwork Cell-mediated Contraction of Collagen Gels
Journal of the Korean Ophthalmological Society
;
: 1548-1553, 2007.
Artículo
en Coreano
| WPRIM
| ID: wpr-105781
ABSTRACT
PURPOSE:
To investigate the effect of nitric oxide (NO) on the contraction of cultured human trabecular meshwork cells (HTMCs).METHODS:
After embedding them into collagen gels, primarily cultured HTMCs were exposed to NO donors, such as sodium nitroprusside (SNP) or S-Nitroso-N-acetylpenicillamine (SNAP), for 1 week at various concentrations, and the contraction of the collagen gels was measured. Cellular survival and NO production were measured with MTT assay and Griess assay, respectively.RESULTS:
Though SNP and SNAP did not significantly affect cellular survival, they markedly enhanced NO production. Both sodium nitroprusside and SNAP inhibited the contraction of collagen gels by about 10% in dose and time-dependent manners (p<0.05).CONCLUSIONS:
NO donors inhibited the contraction of collagen gels in vitro. Thus, NO donors may relax trabecular meshwork and enhance trabecular outflow.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Donantes de Tejidos
/
Malla Trabecular
/
Nitroprusiato
/
Colágeno
/
S-Nitroso-N-Acetilpenicilamina
/
Geles
/
Óxido Nítrico
Límite:
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Ophthalmological Society
Año:
2007
Tipo del documento:
Artículo
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