Proteomic Changes in the Female Rat Bladder Tissue following Bilateral Oophorectomy
Journal of the Korean Continence Society
;
: 134-141, 2009.
Artículo
en Coreano
| WPRIM
| ID: wpr-106841
ABSTRACT
PURPOSE:
To explore the effect of bilateral oophorectomy on the several proteins of female rat bladder via a proteomic approach. MATERIALS ANDMETHODS:
A total of 20 female Sprague-Dawley rats were obtained at 8 weeks of age and were divided randomly into 3 groups a control groups underwent sham operation and bladders were excised 4 weeks and 8 weeks after operation each. Other two groups underwent bilateral oophorectomy and bladders were excised 4 weeks (group 1) and 8 weeks (group 2) after operation each, too. Each group has 5 rats. Conventional proteomics was conducted via high resolution 2-D gel electrophoresis, followed by image analysis and protein identification through mass spectrometry.RESULTS:
Bladder weights were reduced significantly in group 2 as compared with the 8-week control group. A comparison of the bladders of the oophorectomy group subjects with those of the control group subjects demonstrated that the expressions of 11 proteins were altered Eukaryotic translation initiation factor 5A (elF-5A), chaperone grp 75 precursor, actin-depolymerizing factor, contrapsin-like inhibitor 1 precursor, guanine deaminase, actin, peroxiredoxin 2, phosphatidylethanolamine, putative protein kinase, Keratin complex 2 and Enol protein.CONCLUSION:
The roles of 11 proteins are variable. A part of these proteins have a role of cellular apoptosis, acute inflammation, and muscle contraction. It seems that these changes of proteins have an influence on bladder functions and LUTS.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Quinasas
/
Espectrometría de Masas
/
Pesos y Medidas
/
Vejiga Urinaria
/
Ovariectomía
/
Electroforesis en Gel Bidimensional
/
Factores de Iniciación de Péptidos
/
Actinas
/
Ratas Sprague-Dawley
/
Apoptosis
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
/
Femenino
/
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Continence Society
Año:
2009
Tipo del documento:
Artículo
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