Pregnane X Receptor agonist Increases the Expression Levels of the Plasma Membrane Monoamine Transporter
Translational and Clinical Pharmacology
;
: 19-21, 2014.
Artículo
en Inglés
| WPRIM
| ID: wpr-107310
ABSTRACT
We evaluated the effect of the pregnane X receptor agonist, pregnenolone 16 alpha-carbonitrile (PCN) on the expression levels of plasma monoamine transporter (PMAT) in the intestine. Male C57/BL6 mice were divided into two 2 groups mice in the PCN group (n=3) were administered PCN once a day for 4 days, while those in the control group (n=3) received the same volume of vehicle once a day for 4 days. After the mice were killed 24 h after administration of the last dose of PCN or vehicle, and the expression levels of PMAT in the intestine tissues were isolated and measured the expression level of PMAT using immunohistochemical and western blotting analyses. The expression level of PMAT expression levels in the small intestine increased after PCN treatment. These results suggest that the induction of PMAT may play a clinically significant role by increasing intestinal absorption of PMAT substrates such as metformin.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plasma
/
Carbonitrilo de Pregnenolona
/
Membrana Celular
/
Western Blotting
/
Absorción Intestinal
/
Intestino Delgado
/
Intestinos
/
Metformina
Límite:
Animales
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Translational and Clinical Pharmacology
Año:
2014
Tipo del documento:
Artículo
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