Kainic Acid Treatment Increases Ca²⁺-mediated Neurotoxicity in the Mouse Hippocampus / 체질인류학회지
Korean Journal of Physical Anthropology
;
: 71-79, 2016.
Artículo
en Inglés
| WPRIM
| ID: wpr-107701
ABSTRACT
Kainic acid (KA)-induced neuronal cell death is associated with intracellular Ca²⁺ influx. However, it is unknown whether Lyn/Btk pathway is involved in the Ca²⁺-mediated neurotoxicity and neuronal death induced by KA. In the present study, we investigated the altered expression of Ca²⁺-controlled proteins in KA-treated hippocampus. Mice were sacrificed at 24 h after KA (20 mg/kg) systemic injection. We conducted Electroencephalographic (EEG) recording and examined hippocampal alterations by Western blotting and immunostaining in control mice or KA-treated mice. EEG tests showed that KA-treated mice increased seizure frequency and severity compared with control mice during KA-induced seizures. We found that KA decreases hippocalcin and calpain-mediated proteolysis in the hippocampus. In particular, the phosphorylation of Lyn and Btk was increased in KA-treated hippocampus compared to those of control mice. Our findings identify tyrosine kinases such as Lyn/Btk as a critical regulator of Ca²⁺-mediated neurotoxicity in KA-induced seizures.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fosforilación
/
Fosfotransferasas
/
Convulsiones
/
Tirosina
/
Western Blotting
/
Calcio
/
Muerte Celular
/
Electroencefalografía
/
Hipocalcina
/
Proteolisis
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Korean Journal of Physical Anthropology
Año:
2016
Tipo del documento:
Artículo
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