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Immediately transcripted genes in various hepatic ischemia models / 대한외과학회지
Journal of the Korean Surgical Society ; : 298-306, 2012.
Artículo en Inglés | WPRIM | ID: wpr-10839
ABSTRACT

PURPOSE:

To elucidate the characteristic gene transcription profiles among various hepatic ischemia conditions, immediately transcribed genes and the degree of ischemic injury were compared among total ischemia (TI), intermittent clamping (IC), and ischemic preconditioning (IPC).

METHODS:

Sprague-Dawley rats were equally divided into control (C, sham-operated), TI (ischemia for 90 minutes), IC (ischemia for 15 minutes and reperfusion for 5 minutes, repeated six times), and IPC (ischemia for 15 minutes, reperfusion for 5 minutes, and ischemia again for 90 minutes) groups. A cDNA microarray analysis was performed using hepatic tissues obtained by partial hepatectomy after occluding hepatic inflow.

RESULTS:

The cDNA microarray revealed the following interleukin (IL)-1beta expression was 2-fold greater in the TI group than in the C group. In the IC group, IL-1alpha/beta expression increased by 2.5-fold, and Na+/K+ ATPase beta1 expression decreased by 2.4-fold. In the IPC group, interferon regulatory factor-1, osteoprotegerin, and retinoblastoma-1 expression increased by approximately 2-fold compared to that in the C group, but the expression of Na+/K+ ATPase beta1 decreased 3-fold.

CONCLUSION:

The current findings revealed characteristic gene expression profiles under various ischemic conditions. However, additional studies are needed to clarify the mechanism of protection against IPC.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Reperfusión / Daño por Reperfusión / Interleucinas / Ratas Sprague-Dawley / Apoptosis / Adenosina Trifosfatasas / Constricción / Precondicionamiento Isquémico / Análisis de Secuencia por Matrices de Oligonucleótidos / Análisis por Micromatrices Tipo de estudio: Estudio pronóstico Idioma: Inglés Revista: Journal of the Korean Surgical Society Año: 2012 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Reperfusión / Daño por Reperfusión / Interleucinas / Ratas Sprague-Dawley / Apoptosis / Adenosina Trifosfatasas / Constricción / Precondicionamiento Isquémico / Análisis de Secuencia por Matrices de Oligonucleótidos / Análisis por Micromatrices Tipo de estudio: Estudio pronóstico Idioma: Inglés Revista: Journal of the Korean Surgical Society Año: 2012 Tipo del documento: Artículo