DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B elicits polyfunctional CD8+ T cell responses
Clinical and Experimental Vaccine Research
; : 235-243, 2014.
Article
en En
| WPRIM
| ID: wpr-108899
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE: T cell-mediated immune responses, and particularly activation of polyfunctional T cells that simultaneously produce multiple cytokines, are necessary for the control of Mycobacterium tuberculosis. In the present study, we examined if DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B (RpfB) elicits polyfunctional T cell responses in mice. MATERIALS AND METHODS: C57BL/6 mice were immunized intramuscularly three times, at 3-week intervals, with RpfB-expressing plasmid DNA. For comparison, protein immunization was performed with recombinant RpfB in control mice. After immunization, RpfB-specific T cell responses were assessed by interferon-gamma (IFN-gamma) enzyme-linked immunosorbent spot assay and intracellular cytokine staining (ICS), and T cell polyfunctionality was assessed from the ICS data. RESULTS: RpfB DNA immunization induced not only humoral immune responses, but also CD8+ and CD4+ T cell responses. Immunodominant T-cell epitopes were identified within RpfB by assays with overlapping peptides. RpfB DNA immunization elicited a polyfunctional CD8+ T cell response that was dominated by a functional phenotype of IFN-gamma+/TNF-alpha+/IL-2-/CD107a+. CONCLUSION: RpfB DNA immunization elicits polyfunctional CD8+ T cell responses, suggesting that RpfB DNA immunization might induce protective immunity against tuberculosis.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Péptidos
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Fenotipo
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Plásmidos
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Tuberculosis
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ADN
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Linfocitos T
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Factor B del Complemento
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Citocinas
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Inmunización
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Interferón gamma
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Clinical and Experimental Vaccine Research
Año:
2014
Tipo del documento:
Article