Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples
Journal of Veterinary Science
;
: 253-256, 2017.
Artículo
en Inglés
| WPRIM
| ID: wpr-109772
ABSTRACT
There are high levels of co-incidence of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) in porcine tissue. This study established a duplex nested reverse transcriptase polymerase chain reaction (RT-PCR) method that targets the genomic RNA of type 2 PRRSV and the mRNA of PCV2 in infected tissues. The method amplified discriminative bands of 347 bp and 265 bp specific for type 2 PRRSV and PCV2, respectively. The limits of detection of the duplex nested RT-PCR were 10(1.5) TCID₅₀/mL for type 2 PRRSV and 10² infected cells/mL for PCV2. The kappa statistic, which measures agreement between methods, was 0.867, indicating a good level of agreement. This RNA-based duplex RT-PCR approach can be another way to detect type 2 PRRSV and PCV2 simultaneously and with improved convenience.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ARN
/
ARN Mensajero
/
ADN Polimerasa Dirigida por ARN
/
Circovirus
/
Virus del Síndrome Respiratorio y Reproductivo Porcino
/
Síndrome Respiratorio y de la Reproducción Porcina
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Límite de Detección
/
Métodos
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2017
Tipo del documento:
Artículo
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