p53 Mutation in Gastric Carcinoma Detected by PCR - SSCP and Direct - Sequencing / 대한암학회지
Journal of the Korean Cancer Association
;
: 1069-1077, 1998.
Artículo
en Coreano
| WPRIM
| ID: wpr-110369
ABSTRACT
PURPOSE:
p53 gene mutations, one of the most common alterations found in human tumors, has also been detected in gastric carcinoma, and shown to have a crucial and early role in gastric carcinogenesis of intestinal type and mainly associated with tumor progression in the cancer of diffuse type. We tried to investigate the frequency of p53 mutations in 27 gastric carcinomas. MATERIALS ANDMETHODS:
Fresh tumor tissue from a series of gastric carcinoma was screened for p53 mutations by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) with silver staining and confirmed by direct-sequencing in 27 cases of gastric carcinoma. Immunohistochemical method for p53 protein accumulation was also performed in the same cases.RESULTS:
Immunohistochemistry revealed 20 of 27 cases of gastric carcinoma, positive for p53. PCR-SSCP analysis of p53 exons 5-8 detected mobility shift in 4 out of 20 p53-positive tumors; three from exon 5 and the other from exon 7, respectively. DNA sequencing of exon 5 showed CGC to CAC point mutation in one of three cases; exon 7, ATC to AAC point mutation. It seemed that there was no correlation between genetic alterations of p53 gene detected by PCR-SSCP and expression of p53 protein by immunohistochemistry. CONCLUSIOAS Our results suggest that mutations of the p53 gene are rare genetic events in carcinogenesis of gastric carcinomas. There was discrepancy between mutations screened by PCR-SSCP and overexpressions in immunohistochemical staining.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Inmunohistoquímica
/
Reacción en Cadena de la Polimerasa
/
Exones
/
Genes p53
/
Tinción con Nitrato de Plata
/
Análisis de Secuencia de ADN
/
Mutación Puntual
/
Polimorfismo Conformacional Retorcido-Simple
/
Carcinogénesis
Límite:
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Cancer Association
Año:
1998
Tipo del documento:
Artículo
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