Improved immunogenicity of Newcastle disease virus inactivated vaccine following DNA vaccination using Newcastle disease virus hemagglutinin-neuraminidase and fusion protein genes
Journal of Veterinary Science
;
: 21-26, 2016.
Artículo
en Inglés
| WPRIM
| ID: wpr-110769
ABSTRACT
The present study describes the development of DNA vaccines using the hemagglutinin-neuraminidase (HN) and fusion (F) genes from AF2240 Newcastle disease virus strain, namely pIRES/HN, pIRES/F and pIRES-F/HN. Transient expression analysis of the constructs in Vero cells revealed the successful expression of gene inserts in vitro. Moreover, in vivo experiments showed that single vaccination with the constructed plasmid DNA (pDNA) followed by a boost with inactivated vaccine induced a significant difference in enzyme-linked immunosorbent assay antibody levels (p < 0.05) elicited by either pIRES/F, pIRES/F+ pIRES/HN or pIRES-F/HN at one week after the booster in specific pathogen free chickens when compared with the inactivated vaccine alone. Taken together, these results indicated that recombinant pDNA could be used to increase the efficacy of the inactivated vaccine immunization procedure.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Células Vero
/
Virus de la Enfermedad de Newcastle
/
Vacunas Virales
/
Vacunas de Productos Inactivados
/
Proteína HN
/
Chlorocebus aethiops
/
Pollos
/
Proteínas Virales de Fusión
/
Organismos Libres de Patógenos Específicos
/
Vacunas de ADN
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2016
Tipo del documento:
Artículo
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