Damage of Intracytoplasmic Meiotic Spindles in Cryopreserved-Thawed Mouse Oocytes / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 1602-1608, 2001.
Artículo
en Coreano
| WPRIM
| ID: wpr-111971
ABSTRACT
OBJECTIVE:
To investigate the extent of meiotic spindle damages in cryopreserved-thawed mouse mature oocytes.METHODS:
After slow freezing and ultra-rapid thawing using 1.5M dimethylsulfoxide(DMSO), mouse mature oocytes were stained by anti-alpha tubulin monoclonal antibody. The meiotic spindle and chromosomes configuration were assessed using confocal microscope. The influence of time to post-hCG oocytes retrieval (i.e. 12 hrs vs 17 hrs) was also evaluated.RESULTS:
The normal meiotic spindles were observed in 89.8% of post-hCG 12 hrs group, and 80.1% of post-hCG 17 hrs group, and these were significantly lower than that of each unfreezed control. Post-hCG 12 hrs group showed a significantly higher incidence of normal meiotic spindles, compared with post-hCG 17 hrs group.CONCLUSION:
The extent of meiotic spindle damages was significantly increased after cryopreservation in mouse mature oocytes. We proposed that 12 hrs interval of post-hCG oocytes retrieval may be more beneficial.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oocitos
/
Tubulina (Proteína)
/
Criopreservación
/
Dimetilsulfóxido
/
Incidencia
/
Congelación
/
Huso Acromático
Tipo de estudio:
Estudio de incidencia
/
Estudio pronóstico
Límite:
Animales
Idioma:
Coreano
Revista:
Korean Journal of Obstetrics and Gynecology
Año:
2001
Tipo del documento:
Artículo
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