Damage of Intracytoplasmic Meiotic Spindles in Cryopreserved-Thawed Mouse Oocytes / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
; : 1602-1608, 2001.
Article
en Ko
| WPRIM
| ID: wpr-111971
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE: To investigate the extent of meiotic spindle damages in cryopreserved-thawed mouse mature oocytes. METHODS: After slow freezing and ultra-rapid thawing using 1.5M dimethylsulfoxide(DMSO), mouse mature oocytes were stained by anti-alpha tubulin monoclonal antibody. The meiotic spindle and chromosomes configuration were assessed using confocal microscope. The influence of time to post-hCG oocytes retrieval (i.e. 12 hrs vs 17 hrs) was also evaluated. RESULTS: The normal meiotic spindles were observed in 89.8% of post-hCG 12 hrs group, and 80.1% of post-hCG 17 hrs group, and these were significantly lower than that of each unfreezed control. Post-hCG 12 hrs group showed a significantly higher incidence of normal meiotic spindles, compared with post-hCG 17 hrs group. CONCLUSION: The extent of meiotic spindle damages was significantly increased after cryopreservation in mouse mature oocytes. We proposed that 12 hrs interval of post-hCG oocytes retrieval may be more beneficial.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Oocitos
/
Tubulina (Proteína)
/
Criopreservación
/
Dimetilsulfóxido
/
Incidencia
/
Congelación
/
Huso Acromático
Tipo de estudio:
Incidence_studies
/
Prognostic_studies
Límite:
Animals
Idioma:
Ko
Revista:
Korean Journal of Obstetrics and Gynecology
Año:
2001
Tipo del documento:
Article