Withaferin-A Inhibits Colon Cancer Cell Growth by Blocking STAT3 Transcriptional Activity
Journal of Cancer Prevention
;
: 185-192, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-112062
ABSTRACT
BACKGROUND:
Withania somnifera (known as Ashwagandha) is a medicinal plant used in the ayurvedic medicines in India. Withaferin-A, a withanolide derived from the leaf extract of W. somnifera, has been reported to exhibit anti-tumor activity against various cancer cells, such as leukemia, breast cancer and colon cancer cells.METHODS:
We investigated the anti-cancer effects of withaferin-A on the proliferation and migration of human colorectal cancer (HCT116) cells. And we evaluated the effects of withaferin-A on the transcriptional activity of STAT3 and the growth of HCT116 cells in xenograft mouse tumor model.RESULTS:
In the present study, we found that withaferin-A inhibited the proliferation and migration of HCT116 cells in a concentration-dependent manner. Treatment of HCT116 cells with withaferin-A attenuated interleukin-6-induced activation of STAT3, which has been implicated in the development and progression of colon cancer. To examine the effect of withaferin-A on HCT116 cells proliferation in vivo, we generated HCT116 cells xenograft tumors in Balb/c nude mice and treated the tumor bearing mice with or without withaferin-A intraperitoneally. Treatment with withaferin-A exhibited significant decrease in the volume and weight of tumors as compared to untreated controls.CONCLUSIONS:
The present study suggests that withaferin-A holds the potential to be developed as a small molecule inhibitor of STAT3 for the treatment of HCT116.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plantas Medicinales
/
Neoplasias de la Mama
/
Neoplasias Colorrectales
/
Leucemia
/
Colon
/
Neoplasias del Colon
/
Withania
/
Células HCT116
/
Factor de Transcripción STAT3
/
Xenoinjertos
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
/
Humanos
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Journal of Cancer Prevention
Año:
2015
Tipo del documento:
Artículo
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