Genetic diversity of Acanthamoeba isolated from ocean sediments
The Korean Journal of Parasitology
;
: 117-125, 2006.
Artículo
en Inglés
| WPRIM
| ID: wpr-113937
ABSTRACT
Genetic diversity of 18 Acanthamoeba isolates from ocean sediments was evaluated by comparing mitochondrial (mt) DNA RFLP, 18S rDNA sequences and by examining their cytopathic effects on human corneal epithelial cells versus reference strains. All isolates belonged to morphologic group II. Total of 16 restriction phenotypes of mtDNA from 18 isolates demonstrated the genetic diversity of Acanthamoeba in ocean sediments. Phylogenetic analysis using 18s rDNA sequences revealed that the 18 isolates were distinct from morphological groups I and III. Fifteen isolates showed close relatedness with 17 clinical isolates and A. castellanii Castellani and formed a lineage equivalent to T4 genotype of Byers' group. Two reference strains from ocean sediment, A. hatchetti BH-2 and A. griffini S-7 clustered unequivocally with these 15 isolates. Diversity among isolates was also evident from their cytopathic effects on human corneal cells. This is the first time describing Acanthamoeba diversity in ocean sediments in Korea.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Filogenia
/
Variación Genética
/
Océanos y Mares
/
ADN Mitocondrial
/
Acanthamoeba
/
ARN Ribosómico 18S
/
Sedimentos Geológicos
/
Epitelio Corneal
/
Células Epiteliales
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
The Korean Journal of Parasitology
Año:
2006
Tipo del documento:
Artículo
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