Occurrence of a PCR-Positive but Culture-Negative Case for vanB Vancomycin-Resistant Enterococci in Stool Surveillance
Laboratory Medicine Online
;
: 264-268, 2013.
Artículo
en Coreano
| WPRIM
| ID: wpr-114466
ABSTRACT
We present here occurence of PCR-positive but culture-negative for vanB vancomycin-resistant enterococci (VRE) from an enrichment broth of a stool surveillance culture in a patient suffering from Parkinson's disease, who was transferred from a long-term care facility because of aspiration pneumonia. He developed VRE bacteriuria at the hospital day 42. vanA and vanB genes were detected from 6 microg/mL vancomycin-containing BBL Enterococcosel broth (BD), of which color changed to black after overnight incubation, by both Seeplex VRE detection (Seegene, Seoul, Korea) and Anyplex VanR real-time PCR (Seegene). Subculture of an aliquot of the blackened broth on blood agar plate produced only vanA VRE. All of the four subsequent consecutive surveillance cultures for 1 month until discharge at hospital day 75 resulted in PCR-positive but culture-negative for vanB VRE from the enrichment broths. Therefore, the presence of a non-enterococcal intrinsic reservoir bearing vanB is more likely than low burden of vanB VRE. Considering the rare occurrence of vanB VRE in Korea, vanB-positive PCR results from the enrichment broth requires confirmation by microbiological studies.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Enfermedad de Parkinson
/
Neumonía por Aspiración
/
Estrés Psicológico
/
Bacteriuria
/
Ursidae
/
Vancomicina
/
Reacción en Cadena de la Polimerasa
/
Enterococcus
/
Cuidados a Largo Plazo
/
Agar
Tipo de estudio:
Estudio de tamizaje
Límite:
Humanos
País/Región como asunto:
Asia
Idioma:
Coreano
Revista:
Laboratory Medicine Online
Año:
2013
Tipo del documento:
Artículo
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