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Purification and biochemical characterization of two novel antigens from Leishmania major promastigotes
The Korean Journal of Parasitology ; : 287-293, 2007.
Artículo en Inglés | WPRIM | ID: wpr-114843
ABSTRACT
The identification and characterization of antigens that elicit human T cell responses is an important step toward understanding of Leishmania major infection and ultimately in the development of a vaccine. Micropreparative SDS-PAGE followed by electrotransfer to a PVDF membrane and elution of proteins from the PVDF, was used to separate 2 novel proteins from L. major promastigotes, which can induce antibodies of the IgG2a isotype in mice and also are recognized by antisera of recovered human cutaneous leishmaniasis subjects. Fractionation of the crude extract of L. major revealed that all detectable proteins of interest were present within the soluble Leishmania antigens (SLA). Quantitation of these proteins showed that their expression in promastigotes is relatively very low. Considering the molecular weight, immunoreactivity, chromatographic and electrophoretic behavior in reducing and non-reducing conditions, these proteins are probably 2 isoforms of a single protein. A digest of these proteins was resolved on Tricine-SDS-PAGE and immunoreactive fragments were identified by human sera. Two immunoreactive fragments (36.4 and 34.8 kDa) were only generated by endoproteinase Glu-C treatment. These immunoreactive fragments or their parent molecules may be ideal candidates for incorporation in a cocktail vaccine against cutaneous leishmaniasis.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Western Blotting / Cromatografía Líquida de Alta Presión / Leishmania major / Isoformas de Proteínas / Electroforesis en Gel de Poliacrilamida / Antígenos de Protozoos Límite: Animales / Humanos Idioma: Inglés Revista: The Korean Journal of Parasitology Año: 2007 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Western Blotting / Cromatografía Líquida de Alta Presión / Leishmania major / Isoformas de Proteínas / Electroforesis en Gel de Poliacrilamida / Antígenos de Protozoos Límite: Animales / Humanos Idioma: Inglés Revista: The Korean Journal of Parasitology Año: 2007 Tipo del documento: Artículo