Generation, characterization, and application in serodiagnosis of recombinant swine vesicular disease virus-like particles
Journal of Veterinary Science
; : 361-370, 2017.
Article
en En
| WPRIM
| ID: wpr-115771
Biblioteca responsable:
WPRO
ABSTRACT
Swine vesicular disease (SVD) is a highly contagious viral disease that causes vesicular disease in pigs. The importance of the disease is due to its indistinguishable clinical signs from those of foot-and-mouth disease, which prevents international trade of swine and related products. SVD-specific antibody detection via an enzyme-linked immunosorbent assay (ELISA) is the most versatile and commonly used method for SVD surveillance and export certification. Inactivated SVD virus is the commonly used antigen in SVD-related ELISA. A recombinant SVD virus-like particle (VLP) was generated by using a Bac-to-Bac baculovirus expression system. Results of SVD-VLP analyses from electron microscopy, western blotting, immunofluorescent assay, and mass spectrometry showed that the recombinant SVD-VLP morphologically resemble authentic SVD viruses. The SVD-VLP was evaluated as a replacement for inactivated whole SVD virus in competitive and isotype-specific ELISAs for the detection of antibodies against SVD virus. The recombinant SVD-VLP assay produced results similar to those from inactivated whole virus antigen ELISA. The VLP-based ELISA results were comparable to those from the virus neutralization test for antibody detection in pigs experimentally inoculated with SVD virus. Use of the recombinant SVD-VLP is a safe and valuable alternative to using SVD virus antigen in diagnostic assays.
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Texto completo:
1
Índice:
WPRIM
Asunto principal:
Espectrometría de Masas
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Porcinos
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Enfermedad Vesicular Porcina
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Virosis
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Ensayo de Inmunoadsorción Enzimática
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Pruebas Serológicas
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Pruebas de Neutralización
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Microscopía Electrónica
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Certificación
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Western Blotting
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Journal of Veterinary Science
Año:
2017
Tipo del documento:
Article