Gonadotropin Releasing Hormone (GnRH) and GnRH Receptor in Normal Bladder Epithelia and Their Role in Bladder Epithelial Proliferation / 대한비뇨기과학회지
Korean Journal of Urology
;
: 152-157, 2007.
Artículo
en Coreano
| WPRIM
| ID: wpr-116822
ABSTRACT
PURPOSE:
To confirm the production of extra-hypothalamic gonadotropin releasing hormone (GnRH) and GnRH receptor in bladder mucosal epithelia, and a potential role of GnRH on the bladder, normal human bladder tissues, and primary cultured dog bladder mucosal epithelia were studied. MATERIALS ANDMETHODS:
For this study, normal human bladder tissue from 4 patients and primary cultured normal bladder mucosal epithelial cells from 2 dogs were used. For localization of extra-hypothalamic GnRH and the extra-pituitary GnRH receptor, in situ hybridization and immunohistochemical staining were done. To evaluate the roles of exogenous GnRH in bladder mucosal cells, the culture media were supplemented with charcoal stripped serum and 4 different concentrations of GnRH (0, 10(-3), 10(-5) and 10(-7)M). The effect of exogenous GnRH was evaluated using a hemocytometer and fluorescence activated cell sorter (FACS).RESULTS:
GnRH and GnRH receptors, and their mRNA signals were localized in most of the both human bladder mucosal epithelia and dog bladder mucosal epithelia, but not in a few cells. There were no significant GnRH effects on cellular proliferation and cell cycle changes (p<0.05). CONCLISIONS Bladder mucosal epithelium produces GnRH and GnRH receptors, but they do not effect either the proliferation or cell cycle changes. Although the exact function of extra-hypothalamic bladder GnRH is unknown, GnRH and GnRH receptors would be assumed to have unknown autocrine or paracrine relationships with each other.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Vejiga Urinaria
/
ARN Mensajero
/
Ciclo Celular
/
Carbón Orgánico
/
Hormona Liberadora de Gonadotropina
/
Hibridación in Situ
/
Receptores LHRH
/
Medios de Cultivo
/
Proliferación Celular
/
Células Epiteliales
Límite:
Animales
/
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Urology
Año:
2007
Tipo del documento:
Artículo
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