Decreased Expression of Type 5 17beta-Hydroxysteroid Dehydrogenase (AKR1C3) Protein Identified in Human Diabetic Skin Tissue
Annals of Dermatology
;
: 423-427, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-118354
ABSTRACT
BACKGROUND:
Diabetes is characterized by chronic hyperglycemia, and hyperglycemia can increase reactive oxygen species (ROS) production from the mitochondrial electron transport chain. The formation of ROS in cells induces oxidative stress and activates oxidative damage-inducing genes. There is no research on the protein levels of oxidative damage-related genes AKR1C3 in human diabetic skin. We explored the expression of AKR1C3 in diabetic skin compared with normal skin tissue.OBJECTIVE:
To compare the expression of AKR1C3 in normal skin versus diabetic skin.METHODS:
AKR1C3 expression was evaluated by western blotting in 6 diabetic skin tissue samples and 6 normal skin samples. Immunohistochemical staining was carried out to analyze AKR1C3 expression in the 6 diabetic skin tissue samples (July 2009 to December 2011; Department of Plastic and Reconstructive Surgery at Soonchunhyang University Seoul Hospital, Seoul, Korea).RESULTS:
The western blotting showed a significant reduction in AKR1C3 protein expression in diabetic skin tissue compared to normal tissue. Immunohistochemical examination of AKR1C3 showed that it was weakly expressed in all diabetic skin samples.CONCLUSION:
We believe that AKR1C3 is related to diabetic skin in altered metabolic states which elevate ROS production.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oxidorreductasas
/
Plásticos
/
Piel
/
Western Blotting
/
Especies Reactivas de Oxígeno
/
Estrés Oxidativo
/
Transporte de Electrón
/
17-Hidroxiesteroide Deshidrogenasas
/
Hiperglucemia
Límite:
Humanos
Idioma:
Inglés
Revista:
Annals of Dermatology
Año:
2013
Tipo del documento:
Artículo
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