Neonatal Rat Necrotizing Enterocolitis Model Adopting Oral Endotoxin and Hypoxia Exhibits Increased Apoptosis through Caspase-3 Activation
Journal of the Korean Society of Neonatology
;
: 44-52, 2010.
Artículo
en Coreano
| WPRIM
| ID: wpr-118500
ABSTRACT
PURPOSE:
The aim of this study was to develop a model for necrotizing enterocolitis (NEC) in the neonatal rat using endotoxin and hypoxia, a plausible insult in a neonatal intensive care and to investigate the role of apoptosis as the underlying mechanism.METHODS:
Newborn rats were given oral endotoxin and intermittent 8% hypoxia+/-caspase inhibitor. The intestinal histology was evaluated using hematoxylin-eosin staining. Apoptosis was analyzed with TUNEL staining and by measuring the caspase 3 activity in the intestinal lysates. IEC-6 cells were assessed for apoptosis and the expression of Bax, Bcl-2, Fas and FasL was measured after treatment with endotoxin and hypoxia.RESULTS:
Oral endotoxin (5 mg/kg) and exposure to 8% hypoxia of 60-min duration twice induced human NEC-like lesions in the rat intestine. Intestinal tissue revealed increased apoptosis and caspase-3 activity. After caspase inhibitor treatment, the grades of both apoptosis and NEC were significantly reduced. IEC-6 cells exhibited increased apoptosis and caspase 3 activity after endotoxin and hypoxia treatment and significantly increased Bax/Bcl-2 ratio compared to control cells.CONCLUSION:
This neonatal rat model of NEC which was induced by oral endotoxin and intermittent hypoxia showed increased apoptosis of intestinal epithelial cells that was mediated by caspase 3 activation. Our model has a advantage in the study of NEC because the use of much more clinically plausible insults may provide a suitable model for the investigation of its pathophysiology and therapeutic trials.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Cuidado Intensivo Neonatal
/
Apoptosis
/
Enterocolitis Necrotizante
/
Etiquetado Corte-Fin in Situ
/
Células Epiteliales
/
Caspasa 3
/
Imidazoles
/
Intestinos
/
Hipoxia
/
Nitrocompuestos
Límite:
Animales
/
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Society of Neonatology
Año:
2010
Tipo del documento:
Artículo
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