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Gene Expression Profiling of Non-Small Cell Lung Cancer / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment ; : 154-160, 2003.
Artículo en Coreano | WPRIM | ID: wpr-120397
ABSTRACT

PURPOSE:

cDNA microarray provided a powerful alternative, with an unprecedented view scope, in monitoring gene expression levels, and led to the discovery of regulatory pathways involved in complicated biological processes. This study was performed to gain better understanding of the molecular mechanisms underlying the carcinogenesis and progression of lung cancer. MATERIALS AND

METHODS:

Using a cDNA microarray, representing 4, 600 cDNA clusters, we studied the expression profiles in 10 non-small cell lung cancer (NSCLC) samples and the adjacent noncancerous lung tissues form the same patients. The alterations in the levels of gene expression were confirmed by reverse-transcription PCR in 10 randomly selected genes.

RESULTS:

Genes that were differently expressed in the cancerous and noncancerous tissues were identified. One hundred and nine genes (of which 68 were known) and 69 cDNAs (of which 32 were known) were up- and down-regulated in>70% of the NSCLC samples, respectively. In the cancerous tissues, the genes related to the cell cycle, metabolism, cell structure and signal transduction, were mostly up-regulated. Furthermore, we identified a few putative tumor suppressor genes that had previously been proposed by other workers.

CONCLUSIONS:

These results provide, not only a new molecular basis for understanding the biological properties of NSCLC, but also useful resources for the future development of diagnostic markers and therapeutic targets for NSCLC.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fenómenos Biológicos / Transducción de Señal / Expresión Génica / Ciclo Celular / Reacción en Cadena de la Polimerasa / Genes Supresores de Tumor / ADN Complementario / Carcinoma de Pulmón de Células no Pequeñas / Análisis de Secuencia por Matrices de Oligonucleótidos / Perfilación de la Expresión Génica Límite: Humanos Idioma: Coreano Revista: Cancer Research and Treatment Año: 2003 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fenómenos Biológicos / Transducción de Señal / Expresión Génica / Ciclo Celular / Reacción en Cadena de la Polimerasa / Genes Supresores de Tumor / ADN Complementario / Carcinoma de Pulmón de Células no Pequeñas / Análisis de Secuencia por Matrices de Oligonucleótidos / Perfilación de la Expresión Génica Límite: Humanos Idioma: Coreano Revista: Cancer Research and Treatment Año: 2003 Tipo del documento: Artículo