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Evaluation of a serodiagnostic method for tuberculosis by using secreted protein antigens of Mycobacterium Tuberculosis / 결핵
Tuberculosis and Respiratory Diseases ; : 315-323, 2000.
Artículo en Coreano | WPRIM | ID: wpr-122060
ABSTRACT

BACKGROUND:

An immunochromatographic assay (ICT Diagnostics) which facilitates the diagnosis of tuberculosis(TB) by detecting serum antibodies mainly directed against specific 38KDa of Mycobacterium tuberculosis has come into the market. The test consists of a cardboard folding device containing nitrocellulose strip and absorbent pads. The whole procedure is completed within 15 min and does not require any additional equipment. The test has been reported to be sensitive and specific in diagnosing active TB. Thus the test had been evaluated with sera from TB patients and TB-free subjects.

METHOD:

Sera from patients with active pulmonary tuberculosis(40 sputum positives for Mycobacterium tuberculosis, 79 sputum negatives, and 3 extrapulmonary tuberculosis) were obtained from the Double-Cross Chest Clinic of the Korean National Tuberculosis Association (KNTA) in Seoul. The control group consisted of TB-free 68 subjects(21 children under 7 years old and 47 healthy staff members of KNTA).

RESULTS:

Nine out of 68(13.2%) TB-free controls had positive antibody response. Total 106 of 122(86.9%) radiologically active patients had positive antibodies while 16 (13.1%) showed negative reaction. Antibody was detected in 38 of 40(95.0%) sputum positive patients and 68 of 82(82.9%) sputum negative patients who were under the antituberculosis chemotherapy. The sensitivity and specificity were all 87% and the positive predictive value was 92.2% while the negative predictive value was 78.7%, when the prevalence of TB in the sample was 64.2%. It is clear from our results that the detection of antibodies which mainly react with the 38KDa antigen of M. tuberculosis should not be suitable as first-line method of diagnosis, but considered only as an adjunctive test to standard techniques of tuberculosis diagnosis, when considering its high false positivity.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Esputo / Tórax / Tuberculosis / Pruebas Serológicas / Prevalencia / Cromatografía de Afinidad / Colodión / Almohadillas Absorbentes / Diagnóstico / Quimioterapia Tipo de estudio: Estudio diagnóstico / Estudio de prevalencia Límite: Niño / Humanos País/Región como asunto: Asia Idioma: Coreano Revista: Tuberculosis and Respiratory Diseases Año: 2000 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Esputo / Tórax / Tuberculosis / Pruebas Serológicas / Prevalencia / Cromatografía de Afinidad / Colodión / Almohadillas Absorbentes / Diagnóstico / Quimioterapia Tipo de estudio: Estudio diagnóstico / Estudio de prevalencia Límite: Niño / Humanos País/Región como asunto: Asia Idioma: Coreano Revista: Tuberculosis and Respiratory Diseases Año: 2000 Tipo del documento: Artículo