Microbial profile of asymptomatic and symptomatic teeth with primary endodontic infections by pyrosequencing / 대한치과보존학회지
Journal of Korean Academy of Conservative Dentistry
;
: 498-505, 2011.
Artículo
en Inglés
| WPRIM
| ID: wpr-122162
ABSTRACT
OBJECTIVES:
The purpose of this in vivo study was to investigate the microbial diversity in symptomatic and asymptomatic canals with primary endodontic infections by using GS FLX Titanium pyrosequencing. MATERIALS ANDMETHODS:
Sequencing was performed on 6 teeth (symptomatic, n = 3; asymptomatic, n = 3) with primary endodontic infections. Amplicons from hypervariable region of the small-subunit ribosomal RNA gene were generated by polymerized chain reaction (PCR), and sequenced by means of the GS FLX Titanium pyrosequencing.RESULTS:
On average, 10,639 and 45,455 16S rRNA sequences for asymptomatic and symptomatic teeth were obtained, respectively. Based on Ribosomal Database Project Classifier analysis, pyrosequencing identified the 141 bacterial genera in 13 phyla. The vast majority of sequences belonged to one of the seven phyla Actinobacteria, Bacteroidetes, Firmicutes, Fusobacteria, Proteobacteria, Spirochetes, and Synergistetes. In genus level, Pyramidobacter, Streptococcus, and Leptotrichia constituted about 50% of microbial profile in asymptomatic teeth, whereas Neisseria, Propionibacterium, and Tessaracoccus were frequently found in symptomatic teeth (69%). Grouping the sequences in operational taxonomic units (3%) yielded 450 and 1,997 species level phylotypes in asymptomatic and symptomatic teeth, respectively. The total bacteria counts were significantly higher in symptomatic teeth than that of asymptomatic teeth (p < 0.05).CONCLUSIONS:
GS FLX Titanium pyrosequencing could reveal a previously unidentified high bacterial diversity in primary endodontic infections.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Polímeros
/
Propionibacterium
/
Spirochaetales
/
Streptococcus
/
Titanio
/
Bacterias
/
Diente
/
Actinobacteria
/
Genes de ARNr
/
Proteobacteria
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
Journal of Korean Academy of Conservative Dentistry
Año:
2011
Tipo del documento:
Artículo
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