Flow Cytometric Method for Counting Residual Leukocytes / 대한수혈학회지
Korean Journal of Blood Transfusion
;
: 1-7, 2004.
Artículo
en Coreano
| WPRIM
| ID: wpr-122448
ABSTRACT
BACKGROUND:
Simple manual method using a Nageotte hemocytometer counting residual leukocytes [White blood cells(WBC)] in blood components is subjective, labor-intensive, time consuming and variable within and between laboratories. The aim of this study was to evaluate usefulness for flow cytometric method to determinate very low numbers of leukocytes in leukocyte-free blood components.METHODS:
Epics XL-MCL (Beckman Coulter Co.) was used for determination of fluorescence-labelled cells. The DNA in leukocytes was stained using propidium iodide (PI) and leukocytes were automatically analysed by flow cytometer.RESULTS:
Linearity determined over a range of 0.5-50 WBC/muL was a value of r=0.994. The detection limit of this method was 4.4 WBC/muL and accuracy was 86.6% with linearity of r=0.991 over the 5-50 WBC/muL. Reproducibility was CV of 9.1% for 25.8 WBC/muL and 14.7% for 7.1 WBC/muL, respectively.CONCLUSION:
Flow cytometric techniques provide a reproducible and objective tool for counting residual WBC in leukocyte free blood components compared with the Nageotte hemocytometer.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Propidio
/
ADN
/
Límite de Detección
/
Leucocitos
Idioma:
Coreano
Revista:
Korean Journal of Blood Transfusion
Año:
2004
Tipo del documento:
Artículo
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