Genistein activates endothelial nitric oxide synthase in broiler pulmonary arterial endothelial cells by an Akt-dependent mechanism
Experimental & Molecular Medicine
;
: 768-776, 2010.
Artículo
en Inglés
| WPRIM
| ID: wpr-122639
ABSTRACT
Deregulation of endothelial nitric oxide synthase (eNOS) plays an important role in the development of multiple cardiovascular diseases. Our recent study demonstrated that genistein supplementation attenuates pulmonary arterial hypertension in broilers by restoration of endothelial function. In this study, we investigated the molecular mechanism by using broiler pulmonary arterial endothelial cells (PAECs). Our results showed that genistein stimulated a rapid phosphorylation of eNOS at Ser(1179) which was associated with activation of eNOS/NO axis. Further study indicated that the activation of eNOS was not mediated through estrogen receptors or tyrosine kinase inhibition, but via a phosphatidylinositol 3-kinase (PI3K)/Akt-dependent signaling pathway, as the eNOS activity and related NO release were largely abolished by pharmacological inhibitors of PI3K or Akt. Thus, our findings revealed a critical function of Akt in mediating genistein-stimulated eNOS activity in PAECs, partially accounting for the beneficial effects of genistein on the development of cardiovascular diseases observed in animal models.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fosforilación
/
Endotelio Vascular
/
Transducción de Señal
/
Línea Celular
/
Pollos
/
Genisteína
/
Modelos Animales de Enfermedad
/
Activación Enzimática
/
Óxido Nítrico Sintasa de Tipo III
/
Proteína Oncogénica v-akt
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
/
Femenino
/
Humanos
Idioma:
Inglés
Revista:
Experimental & Molecular Medicine
Año:
2010
Tipo del documento:
Artículo
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