Morphine Postconditioning Attenuates ICAM-1 Expression on Endothelial Cells
Journal of Korean Medical Science
;
: 290-296, 2011.
Artículo
en Inglés
| WPRIM
| ID: wpr-123276
ABSTRACT
The purpose of this study is to determine 1) whether morphine postconditiong (MPostC) can attenuate the intercellular adhesion molecules-1 (ICAM-1) expression after reoxygenation injury and 2) the subtype(s) of the opioid receptors (ORs) that are involved with MPostC. Human umbilical vein endothelial cells (HUVECs) were subjected to 6 hr anoxia followed by 12 hr reoxygenation. Three morphine concentrations (0.3, 3, 30 microM) were used to evaluate the protective effect of MPostC. We also investigated blockading the OR subtypes' effects on MPostC by using three antagonists (a micro-OR antagonist naloxone, a kappa-OR antagonist nor-binaltorphimine, and a delta-OR antagonist naltrindole) and the inhibitor of protein kinase C (PKC) chelerythrine. As results, the ICAM-1 expression was significantly reduced in the MPostC (3, 30 microM) groups compared to the control group at 1, 6, 9, and 12 hours reoxygenation time. As a consequence, neutrophil adhesion was also decreased after MPostC. These effects were abolished by coadministering chelerythrine, nor-binaltorphimine or naltrindole, but not with naloxone. In conclusion, it is assumed that MPostC could attenuate the expression of ICAM-1 on endothelial cells during reoxygenation via the kappa and delta-OR (opioid receptor)-specific pathway, and this also involves a PKC-dependent pathway.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Venas Umbilicales
/
Proteína Quinasa C
/
Endotelio Vascular
/
Daño por Reperfusión
/
Transducción de Señal
/
Receptores Opioides
/
Molécula 1 de Adhesión Intercelular
/
Isoformas de Proteínas
/
Células Endoteliales
/
Benzofenantridinas
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Journal of Korean Medical Science
Año:
2011
Tipo del documento:
Artículo
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