RFLP Analysis of a Gene for Blood Coagulation Factor IX in Korean / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 506-512, 2001.
Artículo
en Coreano
| WPRIM
| ID: wpr-123584
ABSTRACT
OBJECTIVE:
The purpose of this study was to investigate the methods for analysis of restriction fragment length polymorphisms of hemophilia B (coagulation factorIX) gene in Korean population.METHODS:
Genomic DNAs were extracted from 40 Korean females. In order to amplify genomic DNAs at the region of the polymorphic sites, two sets of primers (Hha I and Dde I) were synthesized. The primers were named as FIX1, FIX2 for Hha I, and Dde I 59, Dde I 39 for Dde I, respectively. Hha I primers annealed 3'-flanking region of the FactorIX gene and amplified 230 bp long fragment. The PCR fragment (230 bp) treated with Hha I endonuclease produced two fragments (150 bp and 80 bp), when the polymorphic site existed. Dde I primers annealed the region of the first intron of Factor IX gene and amplified 319 bp long fragments. People cases with Dde I polymorphic site are supposed to produce 369 bp long fragment.Results:
It has been found that seven (14 X chromosomes) out of forty individuals showed Hha I polymorphism. However, none of the experimental People cases showed the Dde I polymorphism.CONCLUSIONS:
By the analysis of 80 chromosomes, the PICs calculated from allele frequency of Hha I-RFLP (0.175/0.825) and that of Dde I-RFLP (0.0/1.0) were 0.289=[1-(0.1752+0.8252)] and 0=[1-(02+12)], respectively. From these results, it can be postulated that Hha I and Dde I polymorphisms of the Factor IX gene in Korean exhibited different patterns from those of Caucasian.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Coagulación Sanguínea
/
Polimorfismo de Longitud del Fragmento de Restricción
/
Factores de Coagulación Sanguínea
/
ADN
/
Factor IX
/
Intrones
/
Reacción en Cadena de la Polimerasa
/
Hemofilia B
/
Genes vif
/
Diclorodifenil Dicloroetileno
Límite:
Femenino
/
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Obstetrics and Gynecology
Año:
2001
Tipo del documento:
Artículo
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