Growth Patterns of Human Retinal Pigment Epithelium in Vitro

ABSTRACT

The in vitro growth pattern of the human retinal pigment epithelial cell was carried out from enucleated eye. The cultured cell was observed serially under phase contrast microscopy and stained for light microscopy. The cultured retinal pigment epithelium no longer resembled the original pattern of the retinal pigment epithelium and lost pigment granules as tissue culture progressed. This work has shown that the retinal pigment epithelium was viable and possessed proliferative potency for up to 36 days after seeding without subculture.