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HER-2/neu Oncogene Amplification by Chromogenic in situ Hybridization in 130 Breast Cancers Using Tissue Microarray and Clinical Follow-up Studies
Journal of Korean Medical Science ; : 390-396, 2004.
Artículo en Inglés | WPRIM | ID: wpr-124475
ABSTRACT
Determining of HER-2/neu oncogene amplification has become clinically important for managing breast cancer. Fluorescent in situ hybridization (FISH) and immunohistochemistry (IHC) are currently regarded as the standard methods. Chromogenic in situ hybridization (CISH) was investigated as a new modification with an accurate, sensitive technique. From 1998 to 2002, using CISH and IHC, the amplification and protein expression of the HER-2/neu oncogene were examined using paraffin sections in 130 breast carcinomas and to determine the prognostic role of HER-2/neu for outcome after a follow-up of 24- 64 months. Amplifications by CISH and overexpression by IHC were observed in 28 (22%) and 27 cases (20.8%), respectively. Of the 104 patients, 20 patients (19.2%) with amplification had a shorter disease-free interval (34.9 months vs. 38.0 months in controls) (p=0.372). 15 patients (14.4%) had a disease recurrence, but there is no significant difference between 3 patients amplifying the oncogene and 12 patients without oncogene (20.6 months vs. 19.6 months) (p=0.862). 6 patients (5.8%) of these died. CISH is a useful alternative, particularly for confirming the IHC results. There is no relationship between the early recurrence and the HER-2/neu positive group, but lymph node status was statistically significant.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Pronóstico / Neoplasias de la Mama / Inmunohistoquímica / Estudios de Seguimiento / Sensibilidad y Especificidad / Resultado del Tratamiento / Hibridación Fluorescente in Situ / Hibridación in Situ / Receptor ErbB-2 / Genes erbB-2 Tipo de estudio: Estudio diagnóstico / Estudio observacional / Estudio pronóstico Límite: Adulto / Anciano / Femenino / Humanos Idioma: Inglés Revista: Journal of Korean Medical Science Año: 2004 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Pronóstico / Neoplasias de la Mama / Inmunohistoquímica / Estudios de Seguimiento / Sensibilidad y Especificidad / Resultado del Tratamiento / Hibridación Fluorescente in Situ / Hibridación in Situ / Receptor ErbB-2 / Genes erbB-2 Tipo de estudio: Estudio diagnóstico / Estudio observacional / Estudio pronóstico Límite: Adulto / Anciano / Femenino / Humanos Idioma: Inglés Revista: Journal of Korean Medical Science Año: 2004 Tipo del documento: Artículo