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Activation of acetylcholine receptor elicits intracellular Ca2+ mobilization, transient cytotoxicity, and induction of RANKL expression
International Journal of Oral Biology ; : 119-123, 2016.
Artículo en Coreano | WPRIM | ID: wpr-124491
ABSTRACT
Acetylcholine receptors (AChR) including muscarinic and nicotinic AChR are widely expressed and mediate a variety of physiological cellular responses in neuronal and non-neuronal cells. Notably, a functional cholinergic system exists in oral epithelial cells, and nicotinic AChR (nAChR) mediates cholinergic anti-inflammatory responses. However, the pathophysiological roles of AChR in periodontitis are unclear. Here, we show that activation of AChR elicits increased cytosolic Ca²⁺ ([Ca²⁺]ᵢ), transient cytotoxicity, and induction of receptor activator of nuclear factor kappa-B ligand (RANKL) expression. Intracellular Ca²⁺ mobilization in human gingival fibroblast-1 (hGF-1) cells was measured using the fluorescent Ca²⁺ indicator, fura-2/AM. Cytotoxicity and induction of gene expression were evaluated by measuring the release of glucose-6-phosphate dehydrogenase and RT-PCR. Activation of AChR in hGF-1 cells by carbachol (Cch) induced [Ca²⁺]ᵢ increase in a dose-dependent manner. Treatment with a high concentration of Cch on hGF-1 cells caused transient cytotoxicity. Notably, treatment of hGF-1 cells with Cch resulted in upregulated RANKL expression. The findings may indicate potential roles of AChR in gingival fibroblast cells in bone remodeling.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Periodontitis / Carbacol / Acetilcolina / Expresión Génica / Receptores Colinérgicos / Remodelación Ósea / Citosol / Células Epiteliales / Osteoprotegerina / Fibroblastos Límite: Humanos Idioma: Coreano Revista: International Journal of Oral Biology Año: 2016 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Periodontitis / Carbacol / Acetilcolina / Expresión Génica / Receptores Colinérgicos / Remodelación Ósea / Citosol / Células Epiteliales / Osteoprotegerina / Fibroblastos Límite: Humanos Idioma: Coreano Revista: International Journal of Oral Biology Año: 2016 Tipo del documento: Artículo