Effects of Immune Aggregates, Hydrocortisone and Angiotensin II Receptor Blocker on Interleukin-6 mRNA Expression in Human Mesangial Cells / 대한신장학회잡지
Korean Journal of Nephrology
;
: 199-204, 2002.
Artículo
en Inglés
| WPRIM
| ID: wpr-125461
ABSTRACT
PURPOSE:
Various factors regulate interleukin(IL)-6 expression in mesangial cells (MCs). Immune complexes or angiotensin II(AII) are involved in the development of glomerulonephritis(GN). We evaluated the effects of IgG and IgA aggregates or AII on IL-6 mRNA expression in human MCs and the modulation by losartan, an AT1 receptor blocker, or hydrocortisone.METHODS:
After 48 hours of culture in the presence of sera, IL-6 mRNA expression was analyzed by reverse transcription and polymerase chain reaction(PCR).RESULTS:
Incubation of MCs with IgA or IgG aggregates(100 microgram/mL) as well as AII(10(-7) M) enhanced the ratio of PCR products for IL-6 to beta-actin on densitometric results. The addition of hydrocortisone(0.5 microgrammL) reduced the IgA aggregates-induced IL-6 mRNA expression and losartan(10(-6) M) reduced IgG aggregates- induced IL-6 mRNA expression.CONCLUSION:
These results suggest that IgG and IgA aggregates and AII may induce IL-6 expression in GN which can be partially suppressed by hydrocortisone or AT1 receptor blocker.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Inmunoglobulina A
/
Inmunoglobulina G
/
ARN Mensajero
/
Angiotensina II
/
Angiotensinas
/
Hidrocortisona
/
Receptores de Angiotensina
/
Reacción en Cadena de la Polimerasa
/
Actinas
/
Interleucina-6
Límite:
Humanos
Idioma:
Inglés
Revista:
Korean Journal of Nephrology
Año:
2002
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS