Organotypic slice culture of the hypothalamic paraventricular nucleus of rat
Journal of Veterinary Science
;
: 15-20, 2007.
Artículo
en Inglés
| WPRIM
| ID: wpr-126343
ABSTRACT
Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged period of time. This study evaluated a stationary organotypic slice culture developed for the hypothalamic paraventricular nucleus (PVN) of rat. The results showed that the slice cultures maintain the typical shape of the nucleus, the immunocytochemical signals for oxytocin, vasopressin, and corticotropin-releasing hormone, and the electrophysiological properties of PVN neurons for up to 3 weeks in vitro. The PVN neurons in the culture expressed the green fluorescent protein gene that had been delivered by the adenoviral vectors. The results indicate that the cultured slices preserve the properties of the PVN neurons, and can be used in longterm studies on these neurons in vitro.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oxazinas
/
Núcleo Hipotalámico Paraventricular
/
Hormona Liberadora de Corticotropina
/
Oxitocina
/
Vasopresinas
/
Inmunohistoquímica
/
Adenoviridae
/
Técnicas de Cultivo de Célula
/
Proteínas Fluorescentes Verdes
/
Electrofisiología
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2007
Tipo del documento:
Artículo
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