N epsilon(Carboxymethyl)Lysine-Induced Mesangial Cell Activation / 대한신장학회잡지
Korean Journal of Nephrology
;
: 20-28, 2002.
Artículo
en Coreano
| WPRIM
| ID: wpr-126481
ABSTRACT
BACKGROUND:
Advanced glycation end products (AGE) are independent risk factors in the development and progression of diabetic nephropathy. Receptor for AGE(RAGE) is considered the main receptor involved in AGE-induced cell activation. Galectin-3, another AGE receptor, has recently been found upregulated in mesangial cells(MC) cultured under high glucose and in diabetic rat kidneys. N epsilon(carboxymethyl)lysine(CML) is a well characterized AGE but its role in MC activation is unknown. The present study examined the effects of CML on MC proliferation and extracellular matrix(ECM) secretion.METHODS:
Synchronized rat MC were stimulated with different concentrations of CML-bovine serum albumin(BSA), control BSA, and transforming growth factor-beta(TGF-beta) for up to 72 hours. Cell proliferation was measured by [3H]-thymidine incorporation. Fibronectin, TGF-beta, plasminogen activator inhibitor(PAI)-1 secreted into the media and RAGE and galectin-3 expression in MC were measured by Western blot analysis and ELISA.RESULTS:
1,000 micro /mL of CML-BSA decreased [3H]-thymidine incorporation by MC at 48 hours and 10 ng/mL TGF-beta at 24 and 48 hours. CML-BSA 100 and 1,000 micro /mL, control BSA 1,000 micro /mL, and TGF-beta 10 ng/mL increased fibronectin secretion at 48 hours. CML-BSA up to 1,000 micro /mL did not affect TGF-beta or PAI-1 secretion. TGF-beta 10 ng/mL, however, significantly increased PAI-1 secretion. Cultured MC expressed both RAGE and galectin-3. CML-BSA 100 micro /mL upregulated galectin-3 expression.CONCLUSION:
CML-BSA decreased MC proliferation and increased fibronectin secretion, suggesting that CML may lead to ECM accumulation and glomerulosclerosis in diabetic animals. MC express RAGE and galectin-3 constitutively and CML-induced galectin-3 upregulation may have a role in AGE-induced MC activation.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Furor
/
Ensayo de Inmunoadsorción Enzimática
/
Activadores Plasminogénicos
/
Regulación hacia Arriba
/
Western Blotting
/
Factores de Riesgo
/
Factor de Crecimiento Transformador beta
/
Fibronectinas
/
Inhibidor 1 de Activador Plasminogénico
/
Galectina 3
Tipo de estudio:
Estudio de etiología
/
Factores de riesgo
Límite:
Animales
Idioma:
Coreano
Revista:
Korean Journal of Nephrology
Año:
2002
Tipo del documento:
Artículo
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