Surveillance of Stool Samples for the Presence of Enterobacter sakazakii among Korean People
Yonsei Medical Journal
;
: 1017-1022, 2008.
Artículo
en Inglés
| WPRIM
| ID: wpr-126735
ABSTRACT
PURPOSE:
Enterobacter sakazakii (E. sakazakii) infections are an important cause of life-threatening meningitis, septicemia, and necrotizing enterocolitis in infants. Dried infant formula milk is an important vehicle for E. sakazakii infection. E. sakazakii was isolated in Korea from dried infant formula milk. Although E. sakazakii infection of infants may occur in Korea, its prevalence has not yet been documented. Therefore, we determined the prevalence of E. sakazakii and documented symptoms. MATERIALS ANDMETHODS:
Between March and October 2006, 1,146 stool samples were collected from patients at Uijeongbu St. Mary's Hospital. Each fecal swab was dissolved in 10mL of buffered peptone solution, and enriched culture was streaked onto Druggan-Forsythe-Iversen (DFI) agar. Presumptive E. sakazakii colonies that exhibited a blue-green color during culture on DFI medium were selected. The identity of colonies that developed yellow pigment during culture on TSA was determined using the Vitek system and PCR.RESULTS:
We isolated 4 E. sakazakii strains whose 16S rRNA sequence alignments had a similarity of 99% with those of 3 E. sakazakii ATCC strains.CONCLUSION:
This is the first report on isolation of E. sakazakii from stool samples and to document the symptoms of Korean patients.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ARN Bacteriano
/
Datos de Secuencia Molecular
/
ARN Ribosómico 16S
/
Secuencia de Bases
/
Homología de Secuencia de Ácido Nucleico
/
Farmacorresistencia Bacteriana Múltiple
/
Cronobacter sakazakii
/
Infecciones por Enterobacteriaceae
/
Heces
/
Corea (Geográfico)
Tipo de estudio:
Estudio de tamizaje
Límite:
Adolescente
/
Adulto
/
Anciano
/
Niño
/
Child, preschool
/
Femenino
/
Humanos
/
Lactante
/
Masculino
/
Recién Nacido
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Yonsei Medical Journal
Año:
2008
Tipo del documento:
Artículo
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