Matrix Metalloproteinase-2 Expression of the Injured Anterior Cruciate Ligament in the Knee and Changes in Relation to Signal Transduction Pathways / 대한정형외과연구학회지
Journal of Korean Orthopaedic Research Society
;
: 64-71, 2008.
Artículo
en Coreano
| WPRIM
| ID: wpr-126981
ABSTRACT
PURPOSE:
This study was designed to pursue the basic relationship between MMP-2 and ligament injury via ex vivo stretching of the anterior cruciate ligament (ACL) and its changes by signal transduction pathway. MATERIALS ANDMETHODS:
After femur-ACL-tibia complex was harvested from rats, release of MMP-2 in stretch group and non-stretch group was checked using gelatin zymography. Firstly, authors investigated whether MMP-2 was released from the ligament or not and if so, how long it was released. In addition, the complexes were divided into two groups. In group I, 5 and 12N weights were used to stretch the complex for 10, 30, and 60-minute. In group II, after the ligament complexes were stretched by 15N for 30 minutes, various signal pathway inhibitors such as pertusis toxin, SP600125, PD98059, KT5720, curcumin, and Bay11-7082 were added to their supernatants. MMP-2 activity was evaluated.RESULTS:
MMP-2 was immediately released after ligament injury and its activity was increased in proportion to stretching duration and magnitude. As for the signal pathways, inhibition of JNK, ERK, AP-1, and NF-k beta pathways caused MMP-2 expression to be decreased.CONCLUSION:
It is considered that the release of MMP-2 plays an important role in remodeling process after ACL injury.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Pirroles
/
Sulfonas
/
Pesos y Medidas
/
Flavonoides
/
Carbazoles
/
Transducción de Señal
/
Ligamento Cruzado Anterior
/
Factor de Transcripción AP-1
/
Metaloproteinasa 2 de la Matriz
/
Curcumina
Límite:
Animales
Idioma:
Coreano
Revista:
Journal of Korean Orthopaedic Research Society
Año:
2008
Tipo del documento:
Artículo
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