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Ginseng Total Saponin Improves Podocyte Hyperpermeability Induced by High Glucose and Advanced Glycosylation Endproducts
Journal of Korean Medical Science ; : 1316-1321, 2011.
Artículo en Inglés | WPRIM | ID: wpr-127694
ABSTRACT
Early diabetic nephropathy is characterized by glomerular hyperpermeability as a result of impaired glomerular filtration structure caused by hyperglycemia, glycated proteins or irreversible advanced glycosylation endproducts (AGE). To investigate the effect of ginseng total saponin (GTS) on the pathologic changes of podocyte ZO (zonula occludens)-1 protein and podocyte permeability induced by diabetic conditions, we cultured mouse podocytes under 1) normal glucose (5 mM, = control); 2) high glucose (HG, 30 mM); 3) AGE-added; or 4) HG plus AGE-added conditions and treated with GTS. HG and AGE increased the dextran filtration of monolayered podocytes at early stage (2-8 hr) in permeability assay. In confocal imaging, ZO-1 colocalized with actin filaments and beta-catenin at cell contact areas, forming intercellular filtration gaps. However, these diabetic conditions suppressed ZO-1 immunostainings and disrupted the linearity of ZO-1. In Western blotting, diabetic conditions also decreased cellular ZO-1 protein levels at 6 hr and 24 hr. GTS improved such quantitative and qualitative changes. These findings imply that HG and AGE have an influence on the redistribution and amount of ZO-1 protein of podocytes thereby causing hyperpermeability at early stage, which can be reversed by GTS.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Permeabilidad / Fosfoproteínas / Saponinas / Citoesqueleto de Actina / Línea Celular / Preparaciones de Plantas / Nefropatías Diabéticas / Podocitos / Beta Catenina / Panax Tipo de estudio: Investigación cualitativa Límite: Animales Idioma: Inglés Revista: Journal of Korean Medical Science Año: 2011 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Permeabilidad / Fosfoproteínas / Saponinas / Citoesqueleto de Actina / Línea Celular / Preparaciones de Plantas / Nefropatías Diabéticas / Podocitos / Beta Catenina / Panax Tipo de estudio: Investigación cualitativa Límite: Animales Idioma: Inglés Revista: Journal of Korean Medical Science Año: 2011 Tipo del documento: Artículo